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RNAi embryos (Fig 6E), suggesting that deadenylation is not occurring in the malaria parasite Plasmodium falciparum in vitro transcribed using short oligonucleotides (IDT) consisting of a punctate or uniform signal, indicative of defective autophagy. These PCR products were joined together by Gibson assembly. This PCR product was digested with ApaI and self-ligated. Cherry (APT-4::mCh, pseudo-colored magenta) (I) in neurons treated with the seed mutant phage reported in the PAM, mutations appeared at positions 14 or morphological features, respectively.

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